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Andrea Musacchio Research Papers

Andrea Musacchio (born July 11, 1964) [1] is an Italian structural biologist. He is Max Planck director at the Institute of Molecular Physiology in Dortmund. He is also Honorary Professor at the Center for Medical Biotechnology at the University of Duisburg-Essen. He was named European Molecular Biology Organization member in 2009.[2]

Education and career[edit]

Musacchio received a Laurea in Biology from the University of Rome Tor Vergatasummacum laude in 1990, and earned his Ph.D., summacum laude, from the Heidelberg University in 1995 working at the European Molecular Biology Laboratory. For his postdoctoral studies he joined Stephen C. Harrison laboratory at the Harvard Medical School in Boston. In 1999 he returned to Italy to found his laboratory at the European Institute of Oncology in Milan, where he started working on mechanisms of cell division. He was a European Molecular Biology Organization Young Investigator from 2000-2004. Musacchio moved to Dortmund in 2011 to direct the Department of Mechanistic Cell Biology at the Max Planck Institute for Molecular Physiology[3]

Research[edit]

Musacchio approached structural biology during his Ph.D., contributing to the determination of the first crystallographic structures of the SH3[4] and PH[5] domains. During his post-doctoral training, he was involved in the determination of the clathrin triskelion structure,[6] one of the first studies combining X-ray crystallography and electron microscopy. As an independent scientist, his interests focused on the understanding of the molecular basis of mitosis, with emphasis on the spindle assembly checkpoint. Since the early days, Musacchio's lab adopted a multi-disciplinary approach merging structural biology, biochemistry and cellular biology, this way gaining comprehensive views on protein functions in living cells.[7] He is best known for his work on Mad1/Mad2 complexes,[8][9] which led to the formulation of the template model,[10] and for his contributions to the understanding of the role of the kinase Aurora B, Mps1 and Haspin in mitosis.[11][12][13][14] In 2008, the structure of the Ndc80 complex [15] set the foundation of a new line of his investigations on kinetochore assembly and attachment to microtubules[16][17][18]

Personal life[edit]

Musacchio spent his childhood in Rome, where he grew to be a strong supporter of A.S. Roma. He has started embracing Borussia Dortmund.

He is married to Roberta Palumbo, and is father of two boys, Edoardo and Tommaso.

References[edit]

  1. ^http://www.mpg.de/1040358/molekulare_physiologie_musacchio
  2. ^2009 EMBO Press Release
  3. ^Musacchio A. (2012). "Andrea Musacchio". Current Biology. 22 (14): R552–R554. doi:10.1016/j.cub.2012.06.024. PMID 23012739. 
  4. ^Musacchio, A; Noble, M; Pauptit, R; Wierenga, R; Saraste, M (Oct 29, 1992). "Crystal structure of a Src-homology 3 (SH3) domain". Nature. 359 (6398): 851–5. doi:10.1038/359851a0. PMID 1279434. 
  5. ^Macias, MJ; Musacchio, A; Ponstingl, H; Nilges, M; Saraste, M; Oschkinat, H (Jun 23, 1994). "Structure of the pleckstrin homology domain from beta-spectrin". Nature. 369 (6482): 675–7. doi:10.1038/369675a0. PMID 8208297. 
  6. ^Musacchio, A; Smith, CJ; Roseman, AM; Harrison, SC; Kirchhausen, T; Pearse, BM (Jun 1999). "Functional Organization of Clathrin in Coats: Combining Electron Cryomicroscopy and X-Ray Crystallography". Molecular Cell. 3 (6): 761–70. doi:10.1016/S1097-2765(01)80008-3. PMID 10394364. 
  7. ^Nasmyth, K (Mar 25, 2005). "How do so few control so many?". Cell. 120 (6): 739–46. doi:10.1016/j.cell.2005.03.006. PMID 15797376. 
  8. ^Sironi, L; Mapelli, M; Knapp, S; De Antoni, A; Jeang, KT; Musacchio, A (May 15, 2002). "Crystal structure of the tetrameric Mad1-Mad2 core complex: implications of a 'safety belt' binding mechanism for the spindle checkpoint". The EMBO Journal. 21 (10): 2496–506. doi:10.1093/emboj/21.10.2496. PMC 126000. PMID 12006501. 
  9. ^Mapelli, M; Massimiliano, L; Santaguida, S; Musacchio, A (Nov 16, 2007). "The Mad2 conformational dimer: structure and implications for the spindle assembly checkpoint". Cell. 131 (4): 730–43. doi:10.1016/j.cell.2007.08.049. PMID 18022367. 
  10. ^De Antoni, A; Pearson, CG; Cimini, D; Canman, JC; Sala, V; Nezi, L; Mapelli, M; Sironi, L; Faretta, M; Salmon, ED; Musacchio, A (Feb 8, 2005). "The Mad1/Mad2 complex as a template for Mad2 activation in the spindle assembly checkpoint". Current Biology. 15 (3): 214–25. doi:10.1016/j.cub.2005.01.038. PMID 15694304. 
  11. ^De Antoni, A; Maffini, S; Knapp, S; Musacchio, A; Santaguida, S (Oct 15, 2012). "A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B". The Journal of Cell Biology. 199 (2): 269–84. doi:10.1083/jcb.201205119. PMC 3471222. PMID 23071153. 
  12. ^Santaguida, S; Vernieri, C; Villa, F; Ciliberto, A; Musacchio, A (Apr 20, 2011). "Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction". The EMBO Journal. 30 (8): 1508–19. doi:10.1038/emboj.2011.70. PMC 3102279. PMID 21407176. 
  13. ^Santaguida, S; Tighe, A; D'Alise, AM; Taylor, SS; Musacchio, A (Jul 12, 2010). "Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine". The Journal of Cell Biology. 190 (1): 73–87. doi:10.1083/jcb.201001036. PMC 2911657. PMID 20624901. 
  14. ^Sessa, F; Mapelli, M; Ciferri, C; Tarricone, C; Areces, LB; Schneider, TR; Stukenberg, PT; Musacchio, A (Apr 29, 2005). "Mechanism of Aurora B activation by INCENP and inhibition by hesperadin". Molecular Cell. 18 (3): 379–91. doi:10.1016/j.molcel.2005.03.031. PMID 15866179. 
  15. ^Ciferri, C; Pasqualato, S; Screpanti, E; Varetti, G; Santaguida, S; Dos Reis, G; Maiolica, A; Polka, J; De Luca, JG; De Wulf, P; Salek, M; Rappsilber, J; Moores, CA; Salmon, ED; Musacchio, A (May 2, 2008). "Implications for kinetochore-microtubule attachment from the structure of an engineered Ndc80 complex". Cell. 133 (3): 427–39. doi:10.1016/j.cell.2008.03.020. PMC 4754795. PMID 18455984. 
  16. ^Santaguida, S; Musacchio, A (Sep 2, 2009). "The life and miracles of kinetochores". The EMBO Journal. 28 (17): 2511–31. doi:10.1038/emboj.2009.173. PMC 2722247. PMID 19629042. 
  17. ^Petrovic, A; Mosalaganti, S; Keller, J; Mattiuzzo, M; Overlack, K; Krenn, V; De Antoni, A; Wohlgemuth, S; Cecatiello, V; Pasqualato, S; Raunser, S; Musacchio, A (Feb 20, 2014). "Modular assembly of RWD domains on the Mis12 complex underlies outer kinetochore organization". Molecular Cell. 53 (4): 591–605. doi:10.1016/j.molcel.2014.01.019. PMID 24530301. 
  18. ^Basilico, F; Maffini, S; Weir, JR; Prumbaum, D; Rojas, AM; Zimniak, T; De Antoni, A; Jeganethan, S; Voss, B; van Gerwen, S; Krenn, V; Massimiliano, L; Valencia, A; Vetter, IR; Herzog, F; Raunser, S; Pasqualato, S; Musacchio, A (Jul 8, 2014). "The pseudo GTPase CENP-M drives human kinetochore assembly". eLife. 3: e02978. doi:10.7554/elife.02978. PMC 4080450. PMID 25006165. 

External links[edit]

1. Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova Y, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR & Musacchio A (2016) Structure of the MIS12 complex and molecular basis of its interaction with CENP-C at human kinetochores. Cell, in press.

2. Weir JR, Faesen AC, Klare K, Petrovic A, Basilico F, Fischböck J, Pentakota S, Keller J, Pesenti ME, Pan D, Vogt D, Wohlgemuth S, Herzog F & Musacchio A (2016) Insights from biochemical reconstitution into the architecture of human kinetochores. Nature 537:249-253. doi: 10.1038/nature19333.

3. Klare K, Weir JR, Basilico F, Zimniak T, Massimiliano L, Ludwigs N, Herzog F & Musacchio A (2015) CENP-C is a blueprint for constitutive centromere-associated network assembly within human kinetochores. J Cell Biol. 210:11-22. jcb.201412028.

4. Overlack K, Primorac I, Vleugel M, Krenn V, Maffini S, Hoffmann I, Kops GJ & Musacchio A (2015) A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint. Elife 4:e05269. doi: 10.7554/eLife.05269.

5. Petrovic A, Mosalaganti S, Keller J, Mattiuzzo M, Overlack K, Krenn V, De Antoni A, Wohlgemuth S, Cecatiello V, Pasqualato S, Raunser S & Musacchio A (2014) Modular Assembly of RWD Domains on the Mis12 Complex Underlies Outer Kinetochore Organization. Mol Cell 53:591-605. doi: 10.1016/j.molcel.2014.01.019.

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